Micro-cell chamber and washed human spermatozoa.

With the development of the Makler counting chamber, the reproducibility and accuracy of computer-assisted semen analysis and of manual determination of sperm concentration and sperm motility were significantly enhanced beyond what is possible with other methods, such as the hemocytometer, the Horwell chamber, the Petroff-Hausser chamber, and Chartpak slides. The use of Micro-Cell, a disposable, self-filling counting chamber, gives accurate and reproducible results. The Micro-Cell chamber is found to be more accurate and precise than the Makler chamber in determining sperm concentration. This chamber has become popular for routine semen analysis of specimens after sperm washing for assisted reproductive techniques such as IUI because it eliminates error due to repeated handling, cleaning, and reassembly.